Real-Time Fluorescent and Bioluminescent Fast Imaging

The IVIS Kinetic from Caliper Life Sciences provides a real time, fast im aging system enabling acquisition of biologically relevant events within milliseconds. The IVIS Kinetic can perform both quantitative luminescence and fluorescence as a standard high signal to noise imager and as a high speed imager. The system includes a highly sensitive EMCCD camera for signal enhancement and the ability to reduce exposure times enabling fast kinetics. From the leading optical imaging platform for in vivo analysis, IVIS systems are supported by a range of practical accessories developed through experience in research laboratories worldwide. The IVIS Kinetic offers a light-tight injection port which supporting a syringe injector system enabling real time compound and/or substrate administration.
A removable pull-out tray is included to facilitate pre-imaging animal procedures.

Profª. Responsável: Profª. Drª. Juliana Dreyfuss

Modelo: IVIS Kinetic

Marca: Caliper LifeSciences

Nº de patrimônio 126518 UNIFESP

Financiamento: CT/INFRA-FINEP

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

Suitable for studying chiral molecules of all types and sizes. Often used to analyze biomolecules, Chirascan data contributes to a deeper understanding of biomolecular characteristics, mechanisms and interactions.

Chirascan sets new standards for steady-state circular dichroism spectroscopy.  It incorporates innovative optical design features to maximise light throughput, particularly in the far-UV wavelength region, and a sophisticated digital data acquisition system that facilitates the rapid collection of more accurate and precise CD spectra.  Chirascan’s digital CD spectra acquisition approach ensures that unmodified CD spectra are collected and any post-acquisition smoothing of the CD spectra will be non-distorting and completely reversible. This approach also simplifies the operation – Chirascan is as straightforward to use as a single-beam spectrophotometer

The flow cytometer for your routine cell analysis needs

The BD FACSCalibur™ platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system. Designed with multicolor application support in mind, the BD FACSCalibur flow cytometer provides flexibility to support a wide variety of research and clinical applications

BD FACSCalibur 

Modelo: Becton Dicknson 56874

Marca: Becton Dicknson

Nº. de patrimônio 56.874 UNIFESP

Financiamento: EMU FAPESP

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

Thermo Scientific Microm HM 550

The HM 550 provides a perfect solution for high volume, demanding
clinical and research environments.
Clinical/Research 3
Best-in-Class Sectioning
The Automatic Cryo Approach (ACA) advances specimens to the knife’s edge and stepping motor technology delivers more consistent sections.
With a patented vacutome to flatten sections, Peltier and cryochamber temperature from 0–35°C, best-in-class sectioning is ensured.Low-Fatigue ErgonomicsThe HM 550 allows users to sit or stand and operate controls on the left and right of the arm rests. A convenient LCD display, movable chamber illumination to reduce glare and the ability to motorize sectioning by foot or hand reduce the fatigue associated with other cryostats. Unmatched Protection User safety and infection control permeate the HM 550. The specimen holder al ways stops in the down position for unimpeded access to the tissue section and an emergency stop is accessible by either foot or control panel. The unique debris removal system and state-of-the-art disinfection system ensure user safety

Modelo: HM 550OP

Marca: Microm GMBH

Nº. de patrimonio 73597 UNIFESP

Financiamento: EMU FAPESP

Localização:

INFAR – UNIFESP

Rua Três de Maio,100 - São Paulo, SP.

Descrição do equipamento:

Fabricante: HITACHI – Ano 2011

O espectrofluorímetro de fluorescência HITACHI F-7000, permite a quantificação de alterações de fluorescência, fosforescência e luminescência em cubeta com agitação e aquecimento ou em placa de ELISA.

Características  do equipamento:

- Faixa de comprimento de onda: 200 – 750nm

- Faixa espectral: 1 / 2,5 / 5 / 10 / 20nm

- Exatidão de comprimento de onda: 1nm

- Faixa fotométrica: -9999 a 9999

- Veloc. de varredura: 30, 60, 240, 1200, 2400, 12000, 30000, 60000 nm/min

Acessórios:

• Sistema de controle de temperatura Peltier com suporte de célula. Intervalo de temperatura (-10^oC a 105^oC). O sistema permite análises bioquímicas e celulares que dependem de controle rígido da variação de temperatura.
• Software para medida de variação iônica (IntracellularCationmeasurement) – permite avaliar as modificações iônicas de pH , Ca²⁺, entre outros que são críticas para a identificação das respostas celulares à agonistas e a toxicidade de drogas.
• Adaptador para leitura de placas multiwell (96 poços) – permite realizar ensaios de fluorescência em diferentes condições experimentais, favorecendo a rápida detecção dos efeitos de agonistas e drogas em varias concentrações.

email: tpaschoalin@unifesp.br

telefone: 5576-4450 Ramal 1977

Localização:

Edifício de Pesquisa II – UNIFESP

Rua Pedro de Toledo, 669, 7^o andar, São Paulo, SP.

Formulation studies (liquids and solids) - effects of excipients,

pH and buffers

Determination of secondary structures of de new proteins

Conformational stability and dynamics

Structure in aggregates

Mutation studies

Structural characterization upon environmental effects

Stability studies (thermal and chemical)

Drug delivery

Protein-protein, protein-DNA/RNA and protein-drug interactions

Crystallization condition screening

Modelo: Prota FT-IR Protein Analyzer

Marca: ABB Bomem MB Series

Modelo: SX-20

Marca: Applied Photophysics

Financiamento: AR FAPESP – Ivarne Tersariol

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

SteREO Discovery.V8:

Enhanced Viewing
New high-performance optics – this is the outstanding performance feature that Carl Zeiss has focused on with the development of its latest entry level model in the sophisticated tereomicroscope class. The SteREO Discovery.V8 impresses with enhanced resolution, increased contrast and, most notably, a perceptibly improved stereoscopic impression. As a result, it offers a image brilliance that is without equal in this class of instrument. For a visible increase in information in all biomedical and industrial applica-tions, the time has come for a new view of things.

SteREO Discovery.V8

at a glance:
• Supreme ease of operation and ergonomic viewing posture
• Zoom range of 8 : 1
• Optimized optics design for a visible increasein image information
• Manual focusing drive with adjustable click stops
• Manual and motorized stands with high stability
• Generous specimen space with high working distance
• Illumination and contrast methods based on cold light and LED

Modelo: Stereo Discovery V.8

Marca: Zeiss

Localização:

INFAR – UNIFESP

Rua Três de Maio,100 - São Paulo, SP.

Profª. responsável: Profª. Drª. Mariana Araujo

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

Modelo: EL 101/1

Marca: EletroLab

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

Modelo: EB-60

Marca: Labtrade

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 -  São Paulo, SP.

Modelo: PA 220

Marca: Pachane

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

Profª. responsável: Mariana Araujo

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

Modelo: PA-310

Marca: WR Research Products

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

Alliance 4.7

• Ideal for resolution demanding applications such as 1D quantification, 2D gel, multiplexing,  biofluorescence and western blots

• No compromise: the best of dedicated chemi system and gel-doc gathered in one system

• Massive 16-bit performance

• Dynamic range up to 4.8 orders of magnitude

• Extreme 4.2 Megapixels resolution - 1.1 inch sensor

• Dynamic range up to 4.8 orders of magnitude

• Extremely bright fixed lens (f:0,95)

• Unique twin cooling system

• Patent pending UVI-PURE technology available

• "One touch" fully automated image acquisition program

• USB super-fast connection

FAPESP - Auxílio Pesquisa - Equipamentos Multiusuários Nº 2013/08154-7

Coordenador:
Helena Bonciani Nader

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - Térreo, São Paulo, SP.

The sensitivity of LSM 780 is quite simply outstanding. The GaAsP detector achieves 45 percent quantum efficiency compared to 25 percent typically by conventional PMT detectors. This results in accurate details and contrast-rich images of the challenging specimens you encounter in your live cell imaging.

The system’s illumination and detection design allows you to simultaneously acquire up to ten dyes. You excite any common fluorophore with up to eight different lasers, detecting the signals with the 32 channel GaAsP detector. LSM 780 is so sensitive, the system even allows photon counting.

FAPESP - Auxilio Pesquisa-Regular Nº 2009/52430-3

Coordenadores:

Soraya Soubhi Smaili

Alice Teixeira Ferreira

Helena Bonciani Nader

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - Térreo, São Paulo, SP.

Modelo: AM TIRF MC

Marca: LEICA

Financiamento: JP FAPESP Egdar

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

LEICA SR GSD 3D – 3D LOCALIZATION WITH GROUND STATE DEPLETION

Setting New Standardsin 3D Localization Precision Stochastical optical reconstruction microscopy (GSDIM or dSTORM) can achieve super-resolution images with a lateral resolution of down to 20 nm. However the
z-resolution of a widefield microscope limited resolution around several hundred nanometers. As a pioneer in super-resolution microscopy, Leica Microsystems developed the Leica SR GSD 3D to overcome this limit. This system reaches resolution down to 50 nm in axial direction, setting a new standard in 3D localization precision.

• Widefield super-resolution with ground state depletion
• Maximum resolution in X,Y down to 20 nm
• Maximum resolution in Z down to 50 nm
• The SuMo Stage, with Suppressed Motion technology, minimizes drift for accurate localization of molecules
• Online super-resolution image projection – see results as they are acquired
• Full application flexibility offered by combining super-resolution with TIRF and
• epifluorescence on a multi-purpose live cell imaging system
• Standard fluorochromes can be used – no need to change protocols
• Powerful lasers for the highest flexibility in fluorochrome selection

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - São Paulo, SP.

An automatic, multidetector gamma counter used for detection and quantitative measurement of gamma radiation.

- Ten detector system for high throughput screening
- No scintillation media required in sample vial
- Fully cassette-driven automatic system

The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools

Modelo: 7500 – Real Time PCR System

Marca: Applied Biosystems

Nº. de patrimônio 72.009 UNIFESP

Localização:

INFAR – UNIFESP

Rua Três de Maio, 100 - 6^o andar, São Paulo, SP.

The ABI PRISM®

377 DNA Sequencer 96-lane Upgrade Kit enhances the capabilities of the 377 DNA sequencer to support up to 96 lanes for both Sequencing and GeneScan applications.
Key Features The following list provides an overview of the key features of the 96-lane upgrade.
Increased scan window by 20%, allowing additional lanes to be added without losing sensitivity or increasing scan time Increased number of data collection to 480 channels, allowing data collection of three channels plus two-channel separation per lane Improved Neural Net Tracker, decreasing labor to process gels Increased comb thickness in loading area to 0.4 mm while using 0.2-mm gel for electrophoresis, causing no change in run time Improved comb durability and geometry, allowing easier loading of volumes up to 1.5 μL Added position-based CCD integration with time scaling, allowing collection while accelerating the stage, which minimizes noise Upgraded instruments still run 36-, 48-, and 64-lane gels Hardware Required The following hardware is required to upgrade to 96-lane capability.

ABI PRISM
377-36 or 377XL DNA Sequencer Macintosh®
computer with the following specifications
– Power PC processor
– 32 MB RAM (RAM modules supplied if required)
– Mac OS 8 (supplied in kit)

Modelo: 377 – DNA Sequencer  - ABI Prism

Marca: Applied Biosystems

Nº. de patrimônio 62.522 UNIFESP

 Financiamento: EMU FAPESP

Localização:

INFAR – UNIFESP

Rua Três de Maio,100 - 6^o andar, São Paulo, SP.

Flexible and intuitive chromatography system for fast purification of proteins, peptides, carbohydrates and nucleic acids from microgram to gram levels of target product. These applications are supported by affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). 

SOLICITAÇÃO EM ANÁLISE PELA FAPESP

Formas de agendamento:

telefone: (11) 5576-4444 Ramais: 1188

e-mail: cicazm@gmail.com

Dias disponíveis:

De segunda à sexta-feira, das 08h às 17h, conforme prévio agendamento.

Descrição do Sistema:

Fabricante: GE Healthcare Life Sciences

O Sistema Biacore T200  possibilita o monitoramento de interações intermoleculares em tempo real sem a necessidade de marcação de um dos ligantes. Amostras em solução são injetadas sobre uma superfície contendo um potencial ligante imobilizado, se a amostra interagir com o ligante imobilizado, o índice de refração na interface entre o sensor da superfície e a solução altera proporcionalmente a massa da superfície. O fenômeno ressonância plasmônica de superfície (SPR) é utilizado para detectar estas alterações em tempo real e os dados são apresentados em gráficos denominados de sensorgramas, perfil da resposta do SPR por tempo. O sensorgrama mostra a associação e dissociação do complexo.

Principais aplicações:

O Biacore T200 tem sido utilizado no estudo de interações intermoleculares, incluindo receptores, anticorpos, antígenos, enzimas, fatores de crescimento, glicoproteínas, ácidos nucléicos, drogas, membranas, células e vírus. As informações obtidas por análise em SPR são tanto qualitativas (identificação, verificação de especificidade, mapeamento, mapeamento de epítopo, seletividade, entre outros), como quantitativas (em termodinâmica, estudos cinéticos, determinação de afinidade e de concentração).

Resumidamente, o sistema Biacore T200 permite determinar a especificidade, a cinética e a afinidade de interações moleculares e tem sido amplamente utilizado na caracterização molecular, no desenvolvimento e na procura por novos fármacos sem a necessidade da utilização de marcadores no processo.

email: torquato.bioq@epm.br

telefone: 5576-4444 Ramal 1353

Localização:

Edifício de Pesquisa II – UNIFESP

Rua Pedro de Toledo,669, 8^o andar, São Paulo, SP.

The Tri-Carb 4910TR liquid scintillation counter provides the popular standard features needed for research applications and the versatility to expand for environmental analysis with the optional ultralow-level and alpha/beta discrimination features.

The Vevo 2100 High-Resolution Imaging System comprises a number of technological breakthroughs in the field of high frequency ultrasound; the most notable of which are the development of high frequency linear arrays for the purpose of imaging small animals in a preclinical setting.
Beyond the linear array technologies, the Vevo 2100 also supports a wide variety of new software packages designed to allow quicker and effective imaging of small animal anatomy possible.

Profª. Responsável: Profª. Drª. Juliana Dreyfuss

Modelo: Vevo 2100 Image Systems

Marca: Visual Sonics

Financiamento: CT/INFRA-FINEP

Localização:

INFAR – UNIFESP

Rua Três de Maio,100 - São Paulo, SP.

VICTOR X4 offers several detection methods: luminescence, fluorescence intensity (both top and bottom reading), UV-absorbance (UV-VIS), and time-resolved fluorescence (TRF) technologies.

Specifications: Visible Absorbance: 340-1000 nm; UV Absorbance: 260/280 nm; Luminescence: glow, flash and dual luminescence; Time Resolved Fluorescence (TRF): dual window TRF and dual emission measurements; Fluorescense Intensity: top and bottom fluorescence (340-850 nm; when using optional red-sensitive photomultiplier tube (PMT); Tungsten-halogen lamp and Xenon flash lamp. Compatible with all types of microplates between 1 and 1536 wells, WorkOut Plus MMD software; Dual label measurements; Compatible with Petri plates, slides, filters and PCR plates. Temperature range: up to + 50 °C. 8-position excitation filter wheel for flexible and versatile use of filters. Blue photomultiplier tube (standard). Area scanning up to 100 points/well. Optical system: filter; Plate formats: 96, 384 well plates.